Role of Hemigraphis alternata in wound healing: metabolomic profiling and molecular insights into mechanisms

Hemigraphis alternata (H. alternata), commonly known as Red Flame Ivy, is widely recognized for its wound healing capabilities. However, the pharmacologically active plant components and their mechanisms of action in wound healing are yet to be determined. This study presents the mass spectrometry-based global metabolite profiling of aqueous and ethanolic extract of H. alternata leaves. The analysis identified 2285 metabolites from 24,203 spectra obtained in both positive and negative polarities. The identified metabolites were classified under ketones, carboxylic acids, primary aliphatic amines, steroids and steroid derivatives. We performed network pharmacology analysis to explore metabolite–protein interactions and identified 124 human proteins as targets for H. alternata metabolites. Among these, several of them were implicated in wound healing including prothrombin (F2), alpha-2A adrenergic receptor (ADRA2A) and fibroblast growth factor receptor 1 (FGFR1). Gene ontology analysis of target proteins enriched cellular functions related to glucose metabolic process, platelet activation, membrane organization and response to wounding. Additionally, pathway enrichment analysis revealed potential molecular network involved in wound healing. Moreover, in-silico docking analysis showed strong binding energy between H. alternata metabolites with identified protein targets (F2 and PTPN11). Furthermore, the key metabolites involved in wound healing were further validated by multiple reaction monitoring-based targeted analysis.

Hemigraphis alternata (H.alternata), commonly known as Red Flame Ivy, is widely recognized for its wound healing capabilities.However, the pharmacologically active plant components and their mechanisms of action in wound healing are yet to be determined.This study presents the mass spectrometry-based global metabolite profiling of aqueous and ethanolic extract of H. alternata leaves.The analysis identified 2285 metabolites from 24,203 spectra obtained in both positive and negative polarities.The identified metabolites were classified under ketones, carboxylic acids, primary aliphatic amines, steroids and steroid derivatives.We performed network pharmacology analysis to explore metabolite-protein interactions and identified 124 human proteins as targets for H. alternata metabolites.Among these, several of them were implicated in wound healing including prothrombin (F2), alpha-2A adrenergic receptor (ADRA2A) and fibroblast growth factor receptor 1 (FGFR1).Gene ontology analysis of target proteins enriched cellular functions related to glucose metabolic process, platelet activation, membrane organization and response to wounding.Additionally, pathway enrichment analysis revealed potential molecular network involved in wound healing.Moreover, in-silico docking analysis showed strong binding energy between H. alternata metabolites with identified protein targets (F2 and PTPN11).Furthermore, the key metabolites involved in wound healing were further validated by multiple reaction monitoring-based targeted analysis.
Hemigraphis alternata (H.alternata) belongs to the Acanthaceae family and is distributed in the eastern Malaysia region and adapted to India 1,2 .It is commonly known as red flame ivy, purple waffle plant, and Murikooti.H. alternata is an annual creeping perennial herb growing up to 30 cm tall.The leaves are simple with a toothed margin and arranged in the opposite direction.The leaf blades are silver-coloured on the top and reddish-brown on the bottom, and the stem is erect and reddish-brown.Historically, the leaves of this plant have been used for wound healing and to treat anemia, hemorrhage, dysentery, and kidney stones 3 .In addition, leaf extract can be applied to fresh cuts to help them cure quickly 4 .H. alternata is reported to contain many phytoconstituents such as alkaloids, flavonoids, carbohydrates, steroids, triterpenes, phenols and amino acids 5 .A few studies have reported the antimicrobial, antioxidant, analgesic, and anti-inflammatory activities of H. alternata.Further, an in-silico docking study revealed a higher affinity of compounds from H. alternata for COX-1 (pharmacological targets for anti-inflammatory drugs) compared to the drug aspirin 6 .Moreover, H. alternata also possesses hepato-protective, nephroprotective, anti-ulcer, and anti-diabetic effects 1,[7][8][9] .Recent research has indicated that H. alternata has promising potential for application in the field of biomedicine, particularly as an effective agent for wound healing.A biodegradable biofilm developed from agar/pectin composite containing H. alternata extract showed increased anti-microbial, hydrophilic and anticancer properties 10 .Further, a nanocellulose composite blended with H. alternata extract showed effective anti-microbial properties against diverse bacteria suggesting

LC-MS/MS analysis of H. alternata leaf extracts
Identification of H. alternata metabolites at the MS1 level H. alternata is a medicinal plant used for healing wounds and to treat anemia and hemorrhage.We carried out the LC-MS/MS analysis of an aqueous and ethanolic extract of H. alternata to identify the global metabolite profile and to determine the metabolites involved in wound healing (Fig. 1a).This resulted in the identification of 24,203 spectra from both positive and negative polarities, which corresponds to 2285 metabolites.Metabolite identification at the MS1 level was carried out using MZmine as well as the XCMS Online tool 28 .The metabolites identified from MZmine analysis of aqueous extract included 708 non-redundant metabolites (335 in negative mode and 402 in positive mode) (Supplementary Table S1) and XCMS analysis identified 604 non-redundant metabolites (352 in negative mode and 267 in positive mode) from (Supplementary Table S2).
The metabolites identified from ethanolic extract included 685 non-redundant metabolites (322 in negative mode and 383 in positive mode) from MZmine analysis (Supplementary Table S3) and 548 non-redundant metabolites (325 in negative mode and 236 in positive mode) from XCMS analysis (Supplementary Table S4).MZmine and XCMS Online analysis resulted in the identification of 138 common metabolites and a total of 1157 and 735 metabolites were found unique to MZmine and XCMS Online analysis (Supplementary Table S5).

Identification of H. alternata metabolites at MS/MS level
Metabolite fragment features obtained from MZmine analysis were used to assign metabolites at the MS/MS level with the help of the MS2Compound tool 29 .In the aqueous extract, 250 non-redundant metabolites were assigned at MS/MS level (90 in negative mode and 165 in positive mode) (Supplementary Table S6), whereas in the ethanolic extract, 265 non-redundant metabolites were assigned (117 in negative mode and 149 in positive mode) (Supplementary Table S7).Among the metabolites identified at the MS1 level, 57 metabolites showed MS/MS levels identification (Supplementary Table S8).

Functional enrichment analysis of H. alternata metabolites
The non-redundant metabolites identified from ethanolic and aqueous extract were used to assess their chemical class and biofunctions using the MBROLE tool, and Arabidopsis thaliana was used as a reference database 30 .The chemical classification shows the enrichment of classes including ketones, phenol derivatives, steroids, and carboxylic acids to name a few.The top 10 classes and number of metabolites involved were represented in Fig. 1b, a detailed list is provided in Supplementary Table S9.The highly enriched biofunctions include the component of glycine, serine and threonine metabolism, protein synthesis, component of terpenoid biosynthesis, the component of arginine and proline metabolism, and vasoconstrictor agents (Fig. 1c).
Pathway analysis using the MetaboAnalyst tool shows that H. alternata metabolites were involved in pathways of D-glutamine and D-glutamate metabolism, thiamine metabolism, taurine and hypotaurine metabolism, pyrimidine metabolism, alanine, aspartate, glutamate metabolism, glutathione metabolism, arginine and proline metabolism, and sphingolipid metabolism.Figure 2a represents the major pathways along with their enrichment ratio and p-value.

Protein targets of H. alternata metabolites
Metabolites serve various biological functions as cofactors, allosteric regulators, and protein-complex assembly mediators and influence various biological pathways by interacting with proteins.Tracking the protein-metabolite interaction will help to understand the possible biological role of metabolites.The human protein targets of H. alternata metabolites were predicted by performing BindingDB analysis hoping to discover biologically active

Molecular docking analysis
In this study, we conducted a comprehensive molecular docking analysis to identify H. alternata metabolites involved in the coagulation cascade, focusing particularly on their interactions with essential proteins involved in wound healing pathways.To delve deeper into the potential biological activities of H. alternata metabolites towards their protein targets, we employed an in-depth docking analysis, centering on two crucial wound healing regulators: F2 and PTPN11.Utilizing the Glide 7.7 module of the Schrödinger suite, our study identified a significant active site within the F2/Prethrombin-2 complex (Supplementary Fig. S1).While the technique of active site analysis is well-established, our specific focus on this particular complex and the subsequent docking of H. alternata metabolites provided a unique perspective in our research.The top-ranked site within this complex, which achieved a Site Score of 1.04, was strategically situated at the interface of the F2/Prethrombin-2 complex and served as a pivotal point for our docking study.
In this process, we docked a total of 515 metabolites (264 from the aqueous extract and 251 from the ethanolic extract) revealing a range of binding affinities.The aqueous extract metabolites exhibiting high binding affinity towards F2-Prothrombin included Succinyladenosine (PubChem CID: 20849086), (R)-Kanzonol Y (PubChem CID: 85088559), 3,3′,4′,5,6,7,8-Heptahydroxyflavone (PubChem CID: 44260065), D-Fructose 2,6-bisphosphate (PubChem CID: 105021), and NADH (PubChem CID: 928), with docking scores of − 5.6, − 5.2, − 5.4, 5.9, and − 7.3 kcal/mol, respectively (Fig. 4a-e Likewise, in the exploration of Tyrosine-protein phosphatase non-receptor type 11 (PTPN11), we utilized the protein structure 5EHR from the Protein Data Bank (PDB), complexed with the potent inhibitor SHP099.This foundational analysis enabled us to discern key interaction patterns, particularly hydrogen bonds with Arg111 and Phe113, as well as significant interactions within the PTP domain involving Glu250, essential for high-affinity binding.To validate the accuracy of our docking program, we re-docked SHP099 to the PTPN11 active site (Fig. 5a).The re-docked conformations of the reference inhibitor SHP099 were meticulously compared with its original conformation in the co-crystallized structure.Remarkably, the Root Mean Square Deviation (RMSD) was found to be 0.02 Å, affirming the robustness of our docking algorithm and the reliability of our subsequent analysis.The docking score obtained for SHP099 was − 6.865, providing a benchmark for our interaction studies.
The detailed docking scores, interaction patterns, and Glide scores for these interactions, as presented in Supplementary Tables S11 and S12, highlight the potential functional roles of these metabolites in regulating wound healing and coagulation cascades.The alignment of interaction sites with SHP099 and the comprehensive docking scores for both aqueous and ethanolic extracts underscore the predictive accuracy of our molecular docking analysis.This foundational work paves the way for further experimental validation and opens promising avenues for drug development in wound healing and coagulation cascade regulation.

MRM-based validation of H. alternata metabolites with wound healing potential
Docking results show that the H. alternata metabolites such as NADH, Phosphoribosyl pyrophosphate (PRPP), and Dihydromyricetin (DHY) are the potential compounds directly interacting with key proteins involved in wound healing.So, these metabolites were further validated using MRM analysis.The targeted analysis was carried out for all three metabolites in positive polarity.Using the manual tuning method, the precursor-fragment masses were obtained and 2 to 3 transitions were identified for these metabolites, which were matching to their experimental or predicted spectra available on Competitive Fragmentation Modelling for Metabolite Identification (CFM-ID) and HMDB (Fig. 6a-c).The solvent gradients used for each MRM analysis is provided in Supplementary Table S13.Table 1 lists the precursor mass, transitions, and retention time along with the mass spectrometer parameters-declustering potential (DP), entrance potential (EP), collision energy (CE) and collision cell exit potential (CXP) of NADH, Phosphoribosyl pyrophosphate (PRPP), and Dihydromyricetin (DHY).
Moreover, we identified the presence of curcuminoids such as complex curcumin and curcumin II in H. alternata leaf extract at the MS/MS level.So, MRM was performed for the detection of curcumin in H. alternata leaf extract samples with reference to standard curcumin.The sample was analyzed in technical triplicates.Five MRM transitions were obtained for the curcumin standard (Fig. 6d).The transitions were obtained in positive polarity with a precursor mass of 369 Da for curcumin from H. alternata sample.A total of three transitions were observed in both aqueous (Fig. 6e) and ethanolic extracts (Fig. 6f) of H. alternata sample in MRM mode.The transitions observed were 89 Da, 117.1 Da, 144.9 Da.Curcumin in aqueous extract was quantified by generating a calibration curve with concentrations ranging from 3.4 ng to 875 ng.(Supplementary Fig. S2) The amount of curcumin was found to be 32 ngs per milligram of the leaf extract.The declustering potential (DP), entrance potential (EP), collision energy (CE) and collision cell exit potential (CXP) were optimized for each of the transitions individually as provided in Table 2.

Discussion
Plant metabolites are known to promote wound healing due to their astringent and antimicrobial properties, which appear to be responsible for wound contraction and elevated epithelialization 31,32 .The H. alternata is a known wound healer that is used to treat wounds, and cuts and also directly apply the plant juice on wounds to prevent bleeding 4,33 .The topical application of H. alternata leaf paste promotes wound healing in mice by enhancing wound contraction and epithelialization 34 .Previous research has shown that the hexane extract of H. alternata has shown significant biological activity, including anti-bacterial, anti-oxidant, and anti-elastase activity 35 .However, the mechanism of action and molecular functions of H. alternata metabolites in wound healing is not yet identified.The global and targeted metabolomics approach using mass spectrometry has the potential to detect and characterize metabolites 36 .
The current research aims to explore the active components and constituents of H. alternata through liquid chromatography-mass spectrometry and network pharmacology tools to discover molecular networks associated with the wound-healing process.LC-MS/MS analysis identified several flavonoids, sesterterpene, alkaloids, ketones, diterpenes, sesquiterpenes, and flavonol in H. alternata.Flavonoids and alkaloids are shown to have anti-oxidant, anti-bacterial anti-inflammatory activities, anti-fungal, anti-viral and analgesic properties 37,38 .This study identified various terpenes, which are known for their wide range of medicinal properties including, antimicrobial, anti-inflammatory effect, wound healing, improved blood circulation, and application in treating conditions such as malaria, bacterial infection and migraines 39,40 .This study highlights the presence of several phytochemicals in H. alternata such as carboxylic acids, terpenoids, steroids and steroid derivatives, amino acids, peptides, carbohydrates and carbohydrate conjugates, which were previously reported 41,42 .We evaluated the biofunctions of H. alternata metabolites and the results showed the enrichment of components of various amino acids (serine, threonine, proline, arginine and glycine) metabolism and biosynthesis.Extensive research has been conducted on the involvement of amino acid metabolism in the process of wound healing [43][44][45] .In addition, we identified metabolites involved in vasoconstriction and vasodilation, crucial processes in the initial phase of wound healing (Fig. 1c).The metabolite pathway mapping indicated significant enrichment of pathways associated with amino acid metabolism (D-glutamine and D-glutamate metabolism and arginine metabolism), biosynthesis of glycosphingo lipid, CoA and terpenoid backbone and citrate cycle (TCA) (Fig. 2a).Studies has demonstrated that the plasma concentration of arginine and glutamine decreases during conditions such as injury or wound.These amino acids are essential for various physiological processes, including immune function and tissue repair 46 .
To gain more insights, H. alternata metabolites were subjected to network pharmacology analysis.This allowed us to identify the target proteins of H. alternata metabolites, deciphering their molecular action.The  www.nature.com/scientificreports/interaction between metabolites and proteins is crucial for regulating protein functions, consequently influencing cellular processes 47,48 .Pathway analysis results imply that H. alternata metabolites might influence all stages of wound healing such as hemostasis, inflammation, proliferation and remoldeling 46 by interacting with key proteins associated with these processes.Hemostasis, the initial stage of wound healing is characterized by platelet aggregation and fibrin clot formation 46 .Our results indicate the involvement of H. alternata target proteins in pathways of platelet activation, signaling and aggregation, platelet aggregation, formation of fibrin clots and plug formation.This inference reinforces the potential role of H. alternata extract in halting bleeding.The common proteins involved in these pathways are ABCC4, ADRA2A, ADARA2C, ADRA2B, F2, PTPN11, and YWHAZ.Many reports have demonstrated that B2-adrenoceptors (beta2-ARs) such as ADRA2A, ADARA2C, and ADRA2B are thought to contribute to the modulation of pain regulation of blood flow by vasoconstriction and vasodilation of blood vessels 49,50 .It can also influence inflammation, which is a critical component of the early stages of wound healing 51 .Zhao et al. 52 found that the expression of ADRA2A regulates the expression and secretion of growth factors, cAMP content, and activity of PKA in adipose-derived stem cells in type 2 diabetic (T2D) mice and subsequently influences wound healing.In addition, increased expression of ADRA2A and ADARA2B has been associated with decreased cAMP production leading to impaired re-epithelialization and granulation tissue formation in chronic wounds 53 .F2 (prothrombin) is a key coagulation factor, in active form (thrombin) it converts fibrinogen to fibrin to form a grid to trap red blood cells and platelets.Thrombin is also involved in inflammation, tissue re-modelling and healing 54 .Previous reports have shown that the protein encoded by the PTPN11 (Protein Tyrosine Phosphatase, Non-Receptor Type 11) gene, SHP2 is essential for differentiation, proliferation, metabolism in osteoblasts and epithelial to mesenchymal transition 55,56 .Furthermore, an in-vivo study reported that SHP2 activates HIF-1α by inhibiting 26S proteasome activity and promoting revascularization of wounds 57 .Moreover, YWHAZ (Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Zeta) is known to be involved in signal transduction and regulates a variety of cellular processes including cell division and the stress response.In wound healing, it could be implicated in cell signaling pathways that control cell proliferation, migration, and survival, all of which are vital for effective tissue repair 58 .
H. alternate metabolites may influence inflammation, the second stage of wound healing by interacting with proteins associated with the immune system and interferone signaling (CA1, UBE2N, RORC, PTPN11, PKM and CASP2).The inflammatory response promotes the recruitment of immune cells such as neutrophils, macrophages, dendritic cells and lymphocytes to clear the invading pathogens and also activate the secretion of several proteins that help heal wounds 59 .The pathway analysis also showed significant enrichment of pathways associated with cell proliferation and tissue remodelling such as PI3K cascade, MAPK signaling and RUNX1 regulating transcription of genes involved in differentiation of keratinocytes and signaling by FGFR1/2/3.The proliferative phase of wound healing is characterized by the proliferation, migration and differentiation of keratinocytes.PI3K signaling activates several downstream molecules including AKT, mTOR and GSK3and promotes the expression of VEGF, cyclin D1, and c-My which are essential for cellular proliferation, angiogenesis, metabolism and cellular survival 60 .MAPK signaling and signaling mediated by the RUNX1 are mainly involved in the differentiation and migration of keratinocytes and result in complete epidermal regeneration 61 .In-vivo study reported the role of MAPK signaling in the proliferation and migration of keratinocytes under skin damage 62 .
In-silico docking results show that several metabolites have potential interaction ability with key proteins involved in wound healing such as F2 and PTPN11.Prior to molecular docking, the reference inhibitors and our plant metabolites were prepared using the 'Ligprep' settings in GLIDEv7, optimizing their molecular structures for the subsequent docking process.To evaluate the effectiveness of protein-ligand binding, we utilized an extra precision (XP) scoring function to flexibly dock the compounds within the active sites of F2 and PTNPN11 63 .We further validated the docking methodology by performing re-docking of reference inhibitors of PTPN11 and obtained the RMSD of 0.02 Å affirming the robustness of our docking algorithm.The metabolites such as NADH and phosphoribosyl pyrophosphate (PRPP), an important intermediate in cellular metabolism 64 showed higher docking scores with F2, the protein involved in the coagulation cascade.PTPN11 showed higher docking affinity with flavonoids such as 3,3′,4′,5,6,7,8-Heptahydroxyflavone and dihydromyricetin.Recent studies demonstrated that hydroxyflavone may play a potential role in preventing inflammation 65,66 .Furthermore, dihydromyricetin participates in diverse functions such as anti-inflammatory and antioxidant activities, enhancement of mitochondrial function, and the regulation of autophagy 67,68 .These findings, encapsulating a blend of detailed docking analysis shed light on the promising potential of H. alternata metabolites as therapeutic agents in the coagulation cascade, particularly for wound healing.Nonetheless, it's important to acknowledge that these results are preliminary and predictive, emphasizing the necessity for further experimental studies to verify the biological activity and therapeutic potential of these metabolites.
Among the metabolites identified from in-silico docking, NADH, Phosphoribosyl pyrophosphate, and Dihydromyricetin were further validated by using targeted MRM analysis.Known transitions of these metabolites were successfully detected by the MRM method.NADH is a predominant coenzyme, which plays a major role in the generation of energy, metabolism, reduction-oxidation (redox) reactions, cell survival and death.It is also known to be involved in the regeneration of epithelium during wound healing 69 .Phosphoribosyl diphosphate is an important intermediate in cellular metabolism and an essential component for the synthesis of purine and pyrimidine nucleotides 64 .Dihydromyricetin is a flavonoid with various properties such as antioxidative, antiinflammatory, anticancer, antimicrobial, and lipid and glucose metabolism-regulatory activities 70 .
The global metabolomic profiling of H. alternate plant extract detected the presence of curcuminoids such as complex curcumin and curcumin II, the major turmeric spice polyphenols.Curcumin is known to be involved in several cellular pathways including proinflammatory cytokines, apoptosis, NF-κB, transforming growth factor-β, and STAT3 pathways 71 .Curcuminoids have been shown to participate in the wound healing process by enhancing fibroblast proliferation, granulation tissue formation, and collagen deposition 24,[72][73][74]

Conclusions
The global metabolite profile of H. alternata was performed to investigate the metabolites involved in wound healing.The H. alternata metabolites interacting with F2 and PTPN11 proteins may hold functional imperatives in lead validation, lead optimization, and mechanistic insights for drug development.H. alternata is a promising wound-healing promoter worthy of further studies and clinical evaluation.Thus, the present study confirms the potential value of H. alternata by the presence of various compounds.Given the prominence of metabolite and protein interactions discovered in this study, further investigation of metabolite signatures related to the wound healing process would help to identify and manipulate the key molecules involved in wound healing.A combination of metabolites involved in wound healing identified in this study, as a cocktail may have a future application in wound therapy, which can be tested by in-vivo studies and clinical trials.Further investigation will be needed to elucidate the role of metabolites in wound healing.

Plant material collection and metabolite extraction
H. alternata (Strobilanthus alternata) is grown as a ornamental plant.We collected the leaves of this plant from the cultivated gardens of Yenepoya (Deemed to be) University.Dr. TSKP (Author in this manuscript) has identified it.The identification was also confirmed by Dr. K. R. Chandrashekhar, ex-Professor in the Department of Applied Botany, Mangalore University, India.National Biodiversity Act of 2023, under the Government of India allows the use of any plants for research by Indian citzens.Hence, use of H. alternata (either cultivated or wild), including the collection of plant material, complies with relevant institutional, national, and international guidelines and legislation.The dirt on the leaves was washed off and allowed to dry.Dry leaves were then crumbled into fine powder.The aqueous and ethanolic extracts were prepared by adding distilled water and absolute ethanol (Ratio-1:4) to the leaf powder, respectively, and kept for maceration at 37 °C at 120 rpm for 3 days.Then, the mixture was filtered using filter paper, and the supernatant was concentrated using an oven to get the crude extract.
Mass spectra for aqueous and ethanolic extract of H. alternata were acquired in triplicates.The MS data was acquired through independent positive and negative modes with a mass range from 50 to 1000 and functions based on the EMS-IDA-EPI (enhanced mass spectra-independent data acquisition-enhanced product ion) method.In the MS1 scan, only the top five ions were selected based on their intensity and were further fragmented at the MS/MS level.Electrospray ionization (ESI), Turbo Spray Ion Drive was used as a source.The source temperature was set at 450 °C; ion source voltages were 4500 V in the positive mode − 4500 V in the negative mode; source gas I and gas II at 40 psi and curtain gas at 30 psi.The optimized declustering potentials for positive and negative modes were set at 75 V and − 75 V, respectively.The collision energy (CE) was set as 40 V and − 40 V in positive and negative modes.

Data analysis
Metabolite data analysis was carried out at MS1 and MS/MS levels using MZmine 28 and MS2Compound (https:// sourc eforge.net/ proje cts/ ms2co mpound/) 29 .We also carried out MS1 level analysis using XCMS Online (Forsberg  et al., 2018).MS data obtained in .wiffformat was converted into .mzmLformat using MSConvert, ProteoWizard software 75 .The .mzmL files were processed with MZmine 2.53 as described by Karthikkeyan et al. (2022).The analysis includes the following steps, such as data file import, mass detection, peak picking, peak extension, chromatogram deconvolution, isotope peak grouping, peak alignment across replicates/samples, gap filling, filtering of duplicate peaks and data export.The .mzmL files of aqueous and ethanolic extracts were imported to MZmine and feature detection was performed at MS1 and MS/MS levels.The centroid data with a noise level greater than 1.0E3 was set for MS1 level mass detection and greater than 1.0E1 was set for MS/MS level.The chromatographic peak with a minimum time range of 10 min for peak duration, the amplitude of noise at 1.5E2 a minimum peak height of 1.0E3 and an m/z tolerance of 5 parts per million were selected for chromatogram deconvolution.The chromatogram deconvolution and isotope peak grouping were performed with the noise amplitude and isotopic peak grouper algorithms.The relevant peaks across replicates were aligned with the Joinaligner.Gap filling and filtering of duplicate peaks was carried out with peak finder (multi-threaded) and duplicate peaks filter algorithms.
The metabolite features obtained from MZmine analysis were further used for compound detection at MS1 and MS/MS levels with an in-house built MS2Compound  77 and Human Metabolome Database (HMDB) 78 precursor tolerance was set as 0.05 Da.The fragment tolerance of 0.5 Da and minimum fragment match of 2 was selected for the MS/MS level search.The assigned, non-redundant metabolites with rank 1 were chosen for downstream bioinformatics analysis.Metabolite identification at the MS1 level was also carried out by XCMS Online analysis to improve the identification.The .mzmL files were uploaded through a Java applet, and parameters that matched the instrument setup were selected.The monoisotopic mass obtained from XCMS Online analysis was searched in HMDB for metabolite assignment.The assigned metabolites with the lowest delta ppm value were selected for downstream analysis.

Bioinformatics analysis
The non-redundant metabolites identified in aqueous and ethanolic were analyzed for metabolite classification and biofunction by using MBROLE version 2.0 and pathway enrichment analysis was performed by using MetaboAnalyst 5.0 tool 30 ; Pang et al., 2021).Those with a p-value less than 0.05 were considered significant.
BindingDB analysis was carried out to determine the protein targets of metabolites, the compound names of identified metabolites at the MS/MS level were converted into their SMILES IDs using the PubChem Identifier Exchange Service (https:// www.pubch em.ncbi.nlm.nih.gov/ idexc hange/ idexc hange.cgi) 79 .The SMILES IDs of metabolites were used as inputs for the BindingDB analysis, which predicts the protein-metabolite interactions based on their structural similarity and previously known interactions.A similarity score cut-off of ≥ 0.85 was given to identify the protein targets interacting with the metabolites, and those hit with a score of 1.0 indicate an exact match.UniProt IDs were converted into official gene symbols using the Biological Database network (bioDBnet) 80 .The Gene Ontology analysis was conducted on protein targets of metabolites belonging to Homo sapiens, utilizing the Database for Annotation, Visualization and Integrated Discovery (DAVID) v6.8 81 and pathway enrichment analysis was carried out using the Reactome pathway database (https:// react ome.org/ Pathw ayBro wser/) 82 .

Molecular docking of targets of H. alternata metabolites
The molecular docking analysis was conducted using Glide v7.7, part of the Schrödinger suite (Schrodinger, LLC, New York, NY, 2019-1).To ascertain protein-ligand binding efficacy, we employed an extra precision (XP) scoring function for flexibly docking the compounds with the active sites of the F2/Prethrombin-2 complex (PDB ID: 3K65; Resolution: 1.85 Å) and PTPN11 (PDB ID: 5EHR; Resolution: 1.70 Å).Our procedure entailed several crucial steps: protein preparation, ligand preparation, grid generation, and molecular docking.
Initially, we obtained the three-dimensional crystal structures of the F2/Prethrombin-2 complex and PTPN11 from the Protein Data Bank.For the F2/Prethrombin-2 complex, lacking known inhibitors, we utilized SiteMap for active site prediction.In contrast, for PTPN11, we selected the structure 5EHR, complexed with the potent inhibitor SHP099.SiteMap, a tool within the Schrödinger suite, was instrumental in identifying and analysing potential active sites on the F2/Prethrombin-2 complex, aiding in understanding interaction dynamics and preparing for accurate docking simulations.Before molecular docking, both the reference inhibitors and our plant metabolites underwent preparation using the GLIDEv7 'Ligprep' settings, optimizing the molecular structures for the docking process.We then docked the ligands to the binding sites of the F2/Prethrombin-2 complex and PTPN11 using the Extra Precision (XP) docking algorithm, focusing on assessing binding affinities and interaction patterns.
To validate the accuracy of our docking methodology, a re-docking procedure was performed for the reference inhibitors of PTPN11.The re-docked conformations were rigorously compared with their original conformations in the co-crystallized structures, ensuring the robustness of our docking algorithm and the reliability of our subsequent analyses.The docking scores, G scores, and the nature of the protein-ligand interactions were meticulously reported for the top ligands, providing insights into their binding efficacy and potential as therapeutic agents.

MRM-based validation of H. alternata metabolites with wound healing potential
A stock solution of 1 mg/mL of curcumin (CRMN) from HiMEDIA (RM1449) was prepared with DMSO and stored at − 20 °C.The solvents used were acetonitrile (ACN) of LCMS grade procured from Millipore, water (Milli Q) and formic acid of analytical grade from Sigma.
LC-MS/MS analysis was carried out using a QTRAP 6500 mass spectrometer (SCIEX, Framingham, MA, USA) interfaced with a 1290 Infinity HPLC system (Agilent Technologies).A programmed autosampler injected the samples onto the Zorbax RHP column with the dimensions of 2.1 mm x 50 mm, 2.7 μm (Agilent Technologies, USA).The mobile phases used for the analysis were 0.1% formic acid in water as solvent A and 0.1% formic acid in 90% acetonitrile as solvent B and the flow rate was set to 0.300 mL/min.The total run time was 15 min, and 15 μL of the sample was injected into the column.Data were acquired in positive mode using the MRM scan mode.The Analyst software (version 1.6.3)was used to acquire data, and the Mass range was 50-500 Da.Samples were ionized using the ESI source, gasses were maintained as Gas 1 (GS1) at 35 psi, Ion Source Gas 2 (GS2) at 35 psi, Curtain gas (CUR) at 20.0 psi, ESI source temperature was maintained at 450 °C and collision activated dissociation (CAD) gas at high.The ion spray voltage was set to 5500 V. Analysis of the target metabolites was

Figure 1 .
Figure 1.Biological classification of metabolites identified from H. alternata.(a) Methodolohy workflow employed metabolomics profiling and downstream analysis.(b) Bar diagram depicting the top 10 enriched metabolite classes along with the metabolites count.(c) Represents the highly enriched biofunctions of metabolites identified from H. alternata.

Figure 2 .
Figure 2. Functional enrichment of metabolites identified from H. alternata.(a) The pathway enrichment analysis for metabolites identified in aqueous and ethanolic extract of H. alternata.The X-axis represents the enrichment ratio and the Y-axis represents the pathways.(b) Gene Ontology analysis of protein targets of H. alternata highlighting the enriched biological processes, and (c) molecular functions.The X-axis represents the Gene Ontology terms and the Y-axis represents the − log (p value).

Figure 3 .
Figure 3.The Sankey diagram illustrating the enriched pathways associated with protein targets of H. alternata metabolites and corresponding target genes.

Figure 4 .Figure 5 .
Figure 4. Molecular docking interaction between metabolites and proteins.Docking Poses and interaction patterns of the top hit 5 metabolites from (a-e) aqueous and (f-j) ethanolic extract of H. alternata with Prothrombin (F2).

Figure 6 .
Figure 6.Targeted LC-MS/MS MRM validation for (a) Dihydromyricetin, (b) Nicotinamide adenine dinucleotide hydrogen, (c) phosphoribosyl pyrophosphate.Targeted LC-MS/MS MRM validation of curcumin.(d) Targeted LC-MS/MS MRM spectra of standard curcumin acquired in positive polarity, (e) targeted LC-MS/MS MRM transition spectra of curcumin from aqueous extract of H. alternata leaf, (f) targeted LC-MS/ MS MRM transition spectra of curcumin from ethanolic extract of H. alternata leaf.The MRM transitions as provided in the legend.

Table 1 .
The precursor mass, transitions and polarity of NADH, PRPP, and DHY along with the optimized parameters.

Table 2 .
The precursor and product mass of curcumin in positive polarity along with optimized parameters.